Fermentation Media
The fermentation reaction is a complex transformation of material via the metabolic activity of micro-organisms or via an enzymatic reaction using enzymes obtained from micro-organisms. In 1977, Sheffield's N-Z-Amine™ A (enzymatically hydrolysed casein) was used in pioneering work with Charon phages as vectors for closing DNA in E.coli (Blattner, 1977). Since that time countless researchers have reported the use of N-Z-Amine™ A in both bench level and large-scale production of biopharmaceuticals.
Today, Sheffield Bio-Science is the only media ingredients supplier worldwide that manufactures both peptones and yeast extracts. We provides peptones in the production and/or feed media of 50% of the top 12 blockbuster drugs produced by Escherichia coli (data 2009) and have a long history of being the number 1 global supplier of refined complex nitrogen sources into media for microbial expression of recombinant proteins.
Sheffield has developed a wide range of supplements to meet the nutritional needs of many microorganisms in a broad range of fermentation applications, including:
In our state-of-the-art application lab in Amsterdam (Almere, The Netherlands) we work with our in-house recombinant model systems and customer strains to assist customers with media optimization projects to reach maximum productivity.
Search for your specific strain or contact our technical experts