Feed Supplements should be added to a fed-batch process when the cells are about to enter the stationary phase of growth. The purpose of the timing is to increase the duration of the log phase/stationary phase. This is the point in the cell production cycle where recombinant protein production occurs.
We have developed a range of animal component free (ACF) fed-batch supplements optimized for CHO cells in suspension culture.
AmpliCHO CD Feed - protein-free, chemically defined
Sheff-Pulse I - a protein rich feed supplement
Sheff-Pulse II - a carbohydrate rich feed supplement